ZHU Yunfeng

Professional title:Professor

Office No.:010-51684352


1982-1986:Anhui Normal University, Biology Department,BS

1990-1996:The Institute of Biotechnology in Chinese Military Medical Sciences. MS,PhD

1998-2002:West Virginia University in USA, Visiting Scholar with Post-Doctor position.

Research Field

My projects mainly focus on two sections:

(1) The investigation on non-coding sequences in tumorigenesis, which concerns epigenetic modification, miRNA, siRNA and lncRNA.

(2) The identification of new tumor markers with tumor tissues, cells and circulating blood. Focusing on circulating blood, we develops some special approaches for DNA methylation detection ,which applied for tumor IVD, evaluation of treatment and prognosis prediction.  


PI Grants:

1. The investigation of tumor regents for in vitro tumor diagnosis, selection of candidate drug and treatment evaluation. National “863” Grant(2011AA02A110) 

2. The investigation of regulation of  retrotransposon in tumor cells. Honor Grant of Excellent Scientist in Beijing Jiaotong University.( S11RC00110).  


3. The evaluation for tumor risk by screening of tumor markers in tumor-predisposition people.  The Venture Grant (08060017)

4. Multicenter coordinated detection with FISH approach for prenatal genetic disease and some tumors in China. China National Hygiene Department Grant (WKJ2007-3-001)


Non-PI Grants

1. The biology surveillance for environment contaminant with Cadmium and emergency treatment一Supporting by National Department of Environment Protection ( ES10I00070. 2010.6-2011.5)

2. The key technologies on preparation and optimization of humanized antibodies. Supporting by National Key Items (S11B500041, 2011.3-2013.12)


1. Huanhuan Shi,Chengcheng Lv, Yunfeng Zhu*.Preliminary Study on the Inhibitory Effect of Grape Seed Proanthocyanidins on Colon Cancer Cells.Chinese Journal of Cell Biology. 2019,41(8):1523-1533

2. Huanhuan Shi, Yunfeng Zhu*.Research progress on the role of reactive oxygen species in tumorigenesis and treatmen. Chinese Journal of Pharmacology and Toxicology.,2019,33(5):366-372

3. Yan Liu, Peng Dai, Yunfeng Zhu*.Research progress on the relationship between exosomes and autophagosomes.Chinese Journal of Biological Engineering. 2019,39(6):78-83

4. Yan Liu, Peng Dai, Yunfeng Zhu*.Research progress of exosomes as tumor markers.Chinese Journal of Biological Engineering. 2019,39(8):74-79

5. Liu Shi, Huanhuan Shi, Liangliang Lou, Xiaoning Yuan, Yunfeng Zhu*,L1-ORF1p and Ago2 are involved in a siRNA-mediated regulation for promoter activity of L1-5’UTR,Folia Histochemica et Cytobiologica, 57(2),2019.1-8

6. Yan Chen, Qinglei Zeng,,Xiufang Liu,Junliang Fu,Zhen Zeng, Zhiqin Zhao,Ze Liu, Wenlin Bai, Zheng Dong, Hongjin Liu, Xiaoxia Lu, Yunfeng Zhu*, Yinying Lu*. LINE-1 ORF-1p enhances the transcription factor activity of pregnenolone X receptor and promotes sorafenib resistance in hepatocellular carcinoma cells.  Cancer Management and Research, 2018:10 4421–4438

7. Zhenxiang He, Cuihua Jiang, Jian Zhang, Zhiqi Yin, Zengfang Yin, Yunfeng Zhu*, and Jie Fu*. Neem tree (Azadirachta indica) extract specifically suppresses the growth of tumors in H22-bearing Kunming mice. Z Naturforsch C. 2016(Jun 1), 71(7-8)c: 201–208.

8. Chengcheng Lv, Yunfeng Zhu*.Research progress in the regulation of exosomes in tumorigenesis. Chinese Journal of Pharmacology and Toxicology. Chin J Pharmacol Toxicol,Vol 30,No 7,Jul 2016. PP:777-783

9. Jie Yao1,*Jianxiong Li2,*Peiliang Geng2,*Yi Li3,*Hong Chen3 Yunfeng Zhu2*. Knockdown of a HIF-2α promoter upstream long noncoding RNAimpairs colorectal cancer stem cell properties in vitro through HIF-2α downregulation. OncoTargets and Therapy 2015:8 3467–3474

10. Jie Yao, Baosheng Zhou, Jian Zhang, Peiliang Geng,,Kang Liu,Yunfeng Zhu*, Weijie Zhu. A new tumor suppressor LncRNA ADAMTS9-AS2 is regulated by DNMT1 and inhibits migration of glioma cells. Tumor Biol. DOI 0.1007/s13277-014-1949-2

11. Peiliang Geng, Jie Yao, Yunfeng Zhu*. hOGG1 Ser326Cys polymorphism and lung cancer susceptibility:a meta-analysis. Mol Biol Rep. 2014 Jan 17.  DOI 10.1007/s11033-014-3083-z

12. Qian Yang, Fan Feng, Fan Zhang, Chunping Wang, Yinying Lu, Xudong Gao,Yunfeng Zhu*, Yongping Yang. LINE-1 ORF-1p functions as a novel HGF/ETS-1 signaling pathway co-activator and promotes the growth of MDA-MB-231 cell. Cellular Signalling 25 (2013) 2652–2660。 

13. Yunfeng Zhu*, Fan Feng, Jiyun Yu, Bin Song, Mingming Hu, Xudong Gao,,Yue Wang, and Qing Zhang. L1-ORF1p, a Smad4 Interaction Protein, Promotes Proliferation of HepG2 Cells and Tumorigenesis in Mice. DNA AND CELL BIOLOGY. 2013, 32(9): 1–10.

14. Xiaoning Yuan, Yunfneg Zhu*. Exosome and its roles in tumor regulation. Chinese Journal of biotechnology.2013, 33 (8): 111-117

15. Liangliang Lou, Yunfneg Zhu*。Long non-coding RNA and its regulation in tumor.Chinese Journal of biotechnology. 2013,33 (7): 82-89

16. Yinying Lu, Fan Feng, Yutao Yang, Xudong Gao, Jiajun Cui, Chuanfu Zhang, Fan Zhang, Zhongxian Xu , Jianhui Qv, Chunping Wang , Zhen Zeng , Yunfeng Zhu, Yongping Yang. LINE-1 ORF-1p functions as a novel androgen receptor co-activator and promotes the growth of human prostatic carcinoma cells. Cellular Signalling. 2013, 25: 479–489

17. Yunfeng Zhu*,Guanlin Li.The regulation of retrotransposon, LINE-1, in human genome. Review China agricultural science and technology (Chinese Journal). 2012, 14(4):13-23.

18. Yue Wang, Mingming Hu, Jinqi Yan, Liang Zhang, Yu Wang, Yunfeng Zhu* Jiyun Yu. The effect of L1(ORF1)on cell proliferation by conding- and non-coding RNA. :Bulletin of the Academy of Military Medical Sciences (Chinese Journal). 2011,06:437-441  

19. Mingming Hu, Yunfeng Zhu*。 The expression of LINE-1 ORF1p in E.Coli and preparation of anti-ORF1p polycloning antibody. Chinese Journal of biotechnology. 2010,30 (10):7-11 

20. Xudong Gao,Mingming Hu,Yunfeng Zhu*. The effect of LINE-1 ORF1p on A549 cells by down-regulation. Chinese Journal of biotechnology. 2010,30(6):14-20

21. Yong Wanga, 1, Shouyin Zhangb, 1, Jiyun Yuc, 1, Hui Zhanga, Zhengquan Yuana, Yansong Suna, Ling Zhanga, Yunfeng Zhud, * and Hongbin Songa. An outbreak of Proteus mirabilis food poisoning associated with eating stewed pork balls in brown sauce, Beijing. Food Control. 2010, 21 302–305

22. Yong Wanga, 1, Shouyin Zhangb, 1, Jiyun Yuc, 1, Hui Zhanga, Zhengquan Yuana, Yansong Suna, Ling Zhanga, Yunfeng Zhud, * and Hongbin Songa. An outbreak of Proteus mirabilis food poisoning associated with eating stewed pork balls in brown sauce, Beijing. Food Control. 21 (2010): 302–305

23. Jinqi Yana,1, Rui Jia a,1, Hongbin Songb,1, Ying Liua, Liang Zhanga, Wei Zhanga, Yu Wanga,Yunfeng Zhu c,d,∗, Jiyun Yua,∗∗ A promising new approach of VEGFR2-based DNA vaccine for tumor immunotherapy. Immunology Letters. 2009, 126: 60–66  

24. Yunfeng Zhu*, Quanli Wang, Wangru Xu, Sha Li. The ethanol response gene Cab45 can modulate the impairment elicited by ethanol and ultraviolet in PC12 cells. J. Genet. Genomics. 2008,35:153-161.

25. Yunfneg Zhu*.Calcium binding proteins and its regulation in nerval system diease.  Journal of Biochemistry and molecular biology (Chinese Journal). 2008,24(5):413-418 

26. Shen J, Zhang W, Wu J, Zhu Y. The synergistic reversal effect of multidrug resistance by quercetin and hyperthermia in doxorubicin-resistant human myelogenous leukemia cells. Int J Hyperthermia. 2008, 24(2): 151-159.

27. Yan Sun, Lihua Ding, Hao Zhang, Juqiang Han, Xiao Yang, Jinghua Yan, Yunfeng Zhu, Jiezhi Li, Haifeng Song2 and Qinong Ye.  Potentiation of Smad-mediated transcriptional activation by the RNA-binding protein RBPMS. Nucleic Acids Research, 2006,34: 6314-6326

28. Yanlin Zhang, Yunfeng Zhu*, Sha Li, Junyu, Gao.Effect of down-regulation of LON gene on the proliferation and drug sensitivity of human breast cancer cell line MCF7.Bulletin of the Academy of Military Medical Sciences.2006,30(4):306-309

29. Yaohui Feng, Yunfeng Zhu*, Weijian, Sun.Hedghog signaling pathway and tumorigenesis.Cancer Research.2005,32(12): 796-79

30. Zhong X, Li X, Wang G, Zhu Y, Hu G, Zhao J, Neace C, Ding H, Reed E, Li QQ. Mechanisms underlying the synergistic effect of SU5416 and cisplatin on cytotoxicity in human ovarian tumor cells. Int J Oncol. 2004. Aug; 25 (2): 445-51

31. Runde Jiang, Lixin Zhang, Wen Yue, Yunfeng Zhu, Haojun Lu, Xin Liu, Xintang Qin, Xinyuan Guan, Chunhai Li. Establishment of cisplatin-resistant human nasopharyngeal carcinoma cell line (CNE2/DDP) and screening of resistance-related genes.Chinese Journal of cancer。2003.22(4)337-345

32. Hu,G.,Ryan,S., Zhu Y, Reed,E.,Li,X.,Wang,G.,Li,Q. Vascular endothelial growth factor modulates cisplatin sensitivity in human ovarian carcinoma cells. 2003,Cancer Therapy Vol 1, 1-7

33. Zhu Y,Luo,J. Up-regulation of S3a by EGF in tumor transformation cell model-JB6. Journal of Tumor Marker Oncology. 2003, 18(1):86-99

34. Sun, Y., Lin, H., Zhu Y, Ma, C., Ye, J., Luo, J. Induction or suppression of expression of cytochrome C oxidase subunit II by heregulin beta 1 in human mammary epithelial cells is dependent on the levels of ErbB2 expression. Journal of cellular physiology. 2002,192 (2):225-33.  

35. Zhu Y, Wang, M., Lin, H., Huang, C., Shi, X., Luo, J. Epidermal Growth Factor Up-regulates the transcription of mouse Lon  homology ATP-dependent protease through Extracellular-Signal-Regulated protein kinase- and phosphatidylinol-3 kinase-dependent pathways. Experiment Cell Research. 2002, 280(1):97 -106 .

36. Zhu Y, Luo, J., Barker, J., Hochberg, J., Cilento, E., and Reilly, F.: Up-regulation of truncated L1 and myotubularin by rapid intraoperative tissue expansion in mouse skin. Arch Dermatol Res. 2002, 293(11):560-568

37. Zhu Y*, Evans MI, Estrogen modulates the expression of L-arginine:glycine amidinotransferase in chick liver. Mol Cell Biochem. 2001, 221(1-2):139-45.

38. Zhu Y, Lin H, Li Z, Wang M, Luo J. Modulation of expression of ribosomal protein L7a (rpL7a) by ethanol in human breast cancer cells. Breast Cancer Res Treat. 200, 69(1): 29-38.

39. Zhu Y, Wang, M., Lin, H., Li, Z., and Luo, J. Identification of estrogen-responsive genes in chick liver. Cell Tissue Res. 2001,305(3):357-63.

40. Li, Z., Lin, H., Zhu Y, and Luo, J. Disruption of cell cycle kinetics and cyclin-dependent kinase system by ethanol in cultured cerebellar granule progenitors. Dev Brain Res. 2001,132(1):47-58.

41. Zhu Y, Lin, H., Li, Z, Wang, M., and Luo, J. Up-regulation of transcription of smooth muscle myosin alkali light chain by ethanol in human breast cancer cells. International journal of Oncology. 2001, 18: 1299-1305.


National Innovation Patients:

  1. The kit and oligonucleotides for cfDNA detection in plasma.  No:201310741282.6;(Approved)
  2. The kit and primer set for detecting gene expression of cytosine deaminase and related molecules in cell-free DNA of peripheral blood.No.201510593883.6 (Approved) 
  3. The kit and its oligonucleotide pairs for detecting P53 gene fragments in free DNA from peripheral blood. No.201810536403.6 (Approved)
  4. The kit for detection of cytosine deaminase and related genes in cfDNA and its application.No. 201810536401.7  (Approved)
  5. A quantitative PCR method for identifying differences in DNA epigenetic modification. No. 201810536332.x(Review)

Social Appointments

The Reports in International Conferences

  1. Zhu Yunfeng. The role of peripheral blood free DNA as a new tumor marker in tumor screening and efficacy evaluation.2015 Biomarker Symposium.2015,09-17—09-18(Host and Reporter)
  2. Zhu Yunfeng. “Retrotransposon, L1, the Culprit for Tumorigenesis in Genomics”--- World Cancer Congress 2010,Singapore Expo. 2010, Jun (22-25).
  3. Zhu Yunfeng. “Mitochondria, a promising target for molecular diagnosis in tumor” ----Chinese Mit’2006- Mitochondria and Health, an international conference of Mitochondrial Biomedicine. Wenzhou Medical College, China. 2006, Nov (2-6)。
  4. Zhu Yunfeng, An EGF response gene- Lon Homology ATP-dependent Protease has an important role in the process of tumor formation. The International Tumor Biology Conference & 21st International Tumor Marker Conference in Asia Pacific Area.Xi’An, China.  2004, Aug. (21-25)
  5. Zhu Yunfeng, A ribosomal protein S3a with its function beyond structure protein can be up-regulated by EGF in tumor transformation cell model--JB6. The first conference on tumor targeting therapy .Hosted by China Bio-medicine Engineering Association. Sanya, China, 2003, Nov.
  6. Zhu Y, Lin, H., Li, Z, Wang, M., and Luo, J. Up-regulation of transcription of smooth muscle myosin alkali light chain by ethanol in human breast cancer cells. 24th Annual Scientific Meeting of the Research Society on Alcoholism. Hilton Montreal, Canada, 2001  
  7. Zhu Y, Luo, J., Barker,J., Hochberg, J., Cilento,E., and Reilly, F. Up-regulation of L1 and myotubularin by rapid intraoperative tissue expansion in the mouse skin. The 7th World Congress for Microcirculation, Sydney, Australia, 2001
  8. Barker,J., Cilento, E., Hochberg, J., Luo, J., Reilly, F., and Zhu Y: Stretch-responsive gene up-regulated in the mouse skin. The XXXIV International Congress of Physiological Sciences, Christchurch, New Zealand.
  9. Hochberg, J., Zhu Y, Luo, J., Barker,J., Cliento,E., and Reilly, F., Rapid intraoperative tissue expansion up-regulates a stretch-responsive gene in mouse skin. Proceedings of the Annual Meeting of ASPS/PSEF/ASMS, Orlando, FL, 2001
  10. Zhu Y, Lin, H., Li, Z., and Luo, J. Modulation of cell proliferation and gene expression by ethanol in human breast cancer cells. AACR 92nd Annual Meeting. 2000.